Experts in recombinant protein services. We are a multidisciplinary team of almost 30 people including biologists, biotechnologists, chemical and process engineers with over 15 years of experience overexpressing proteins. 53Biologics works in a quick, proactive and efficient way, always ensuring very high-quality standards.


We are experts in the optimization of all bioprocess stages. The biologist and biotechnologist who composed our Molecular Biology & Microbiology team have many years of experience working with all the platforms commonly used to express proteins, from well-known microbial hosts like E. coli or Pichia pastoris to mammalian or insects cell lines.

Furthermore, we have acquired expertise with other innovative solutions: bacteria like Bacillus,yeasts like Saccharomyces or even fungal platforms like Trichoderma or the revolutionary C1.



We have also developed and have available many tools in-house to improve and increase the protein expression levels of most microbial strains and cell lines. With this portfolio, we can easily demonstrate that there is no challenge too complex for us.



Our chemical and process engineers are experts in the up and downstream process development and improvement.

We can help you to reduce your production costs by improving your fermentation conditions, optimizing your culture media and helping you to scale-up efficiently your process. To do this, we have several stainless steel and single use reactors from 1 to 100 liter-reactors and a hundred-liter reactor fully monitored.

Process development also includes the downstream of the culture’s broths, clarification by centrifugation, cell rupturing, tangential flow filtrations by means microfiltration or ultrafiltration that are combined with diafiltration and chromatographic processes.

Upstream and Downstream processing is then simplified, characterized, optimized and registered.

We always keep very close contact with our clients with regular follow-ups where our project managers would gather and communicate all the results obtained and discuss the next steps of the project. Our customers are also always welcomed to participate on site on the discussion of the results. We believe this close communication is vital to ensure that the product IMPD would include all the relevant information.

We care about the success of your projects as if they were our own. No matter the size of the project or the complexity of the challenge, our experts will find and execute the precise solution to hit your target.