This small Gram-negative Enterobacteriaceae has revolutionized the Molecular Biology and Biochemistry relegating to the past the large amount of animal and plant tissues processing to obtain small amounts of protein.
There are uncountable molecular tools and protocols to transform E. coli and to obtain recombinant proteins from it, like a huge catalog of expression plasmids combining promoters, origin of replications, signal sequences (i.e to periplasmic space or to inclusion bodies), a great number of engineered strains and well known strategies to culture E. coli in large fermenters.
However, sometimes these approaches are dispersed and only available at the theoretical level, it is difficult to find a strong catalogue of updated solutions. The cloning and production, even being straightforward processes, are also minefields that could stop your developments, poor growth of the host, inclusion bodies (IB) if you want soluble overexpression, protein inactivity, low yields or even not obtaining any protein and many more…
Starting with the gene sequence of your protein of interest, 53Biologics could guide you with the selection and troubleshooting of the suitable expression vector or base strain and the best production and downstream strategy, to achieve acceptable yields obtaining your purified protein.