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53Biologics operates in 2 buildings located in the Boecillo Technological Park in Valladolid, Spain

Development & Manufacturing Facilities

With more than 2200 m², our state-of-the-art facilities include Cell Culture Suites, capabilities for Process Development and non-GMP and GMP manufacturing plant.

GMP warehouse and Analytical laboratories

More than 1200 m² of which 400 m² correspond to our cutting-edge analytical labs. The rest space is used as our GMP storage with cold rooms and climatic chambers.

We are experts in Decoding Biologics Production and as a customer-focused CDMO, we adapt to your needs.

Development and Manufacturing Facilities

Cell Culture Suites
We have different rooms for the operations with microbial and mammalian cell lines.
  • Microbial: in our facilities we have dedicated rooms with cutting-edge equipment for research and development projects involving bacteria, yeast and fungal cell banks.
  • Mammalian: Mammalian cell culture suites are nowadays located in a separated building, the bioincubator of Boecillo Technology Park. Its suites are equipped with advanced equipment for research and development activities related with cell line generations.
Process Development Facilities
We have different pilot plants for:

  • Upstream Process Development: From flask to several 1 L, 5 L and up to 30 L, wave and bench stirred bioreactors, from stainless steel vessels to single use, for mammalian and microbial production cultures.
  • Downstream Process Development: Equipped with the most advanced technology and units operations, discontinuous and continuous disk and stack centrifuges, high pressure homogenizers, sonicators, depth filtration skids, tangential flow filtration equipment for different membranes from micro to ultrafiltration, chromatography equipment and auxiliary tanks.

Manufacturing Lines

We have different lines:

  • Line 0: GMP Cell Banking 
  • Line 1: Research grade Production up to 500 L for Protein & pDNA
  • Line 2: GMP Production up to 200 L for Protein & pDNA
  • Line 3: GMP Protein Production up to 2000 L
  • Line 4: RNA Production (in current development)

53Biologics provide you flexible manufacturing capacities and short turnaround times.

GMP warehouse and Analytical laboratories

More than 1200 m² of which 400 m² correspond to our cutting-edge analytical labs. The rest space is used as our GMP storage with cold rooms and climatic chambers.

More than 1200 m² of which 400 m² correspond to our cutting-edge analytical labs. The rest space is used as our main storage rooms (from -80ºC to, -20ºC, 4ºC, 15-25ºC), climatic chambers, reception and shipments decks, GMP storage and dispensing areas.

Our CDMO services guarantee the delivery of your biologic in time, within budget and in the quality standards of your choice

    Discover more about 53Biologics services

    Plasmid DNA Services

    We provide plasmid DNA production services to fulfill your requirements and accelerate your advanced therapies production.
    Learn More

    mRNA Services

    We cover the entire mRNA manufacturing value chain, from DNA template generation and linearization to RNA purification.
    Learn More

    Protein Services

    From finding the most suitable host to contract manufacturing, 53Biologics will make the most of your protein of interest.
    Learn More