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CDMO Services for Microbial Proteins
Scientific depth and adaptable manufacturing support for microbial proteins, from strain development to GMP production. A partnership shaped around your protein and designed to grow with your program.
Our Expertise in Microbial Hosts
E. coli
At 53Biologics we provide engineered E. coli strains, optimized plasmids and high-throughput workflows to maximize protein productivity while minimizing timelines to manufacturing.
High productivity
Extensive track record
Ideal for non-glycosylated proteins
P. pastoris
Our expertise in yeast expression systems, particularly P. pastoris, allows us to produce recombinant proteins with high yield and scalability.
Ability to secrete proteins
Easily scalable from research to GMP production
IP-free toolkits reduce licensing costs
Others
53Biologics can work with other microbial strains like Bacillus or fungal strains (Trichoderma, Aspergillus or the revolutionary Myceliophthora thermophila, nicknamed C1) that have demonstrated high performance in protein productivity, complexity and efficiency.
Ability to secrete proteins
Fungal strains are suitable for complex proteins
Pioneer CDMO in fungal platforms
3
Manufacturing lines
~11.6
Years of average team experience
2/3
Repeat clients
150
Recombinant proteins released
Overview
Is Microbial Expression the Right Choice for Your Protein?
Every recombinant protein has different structural and functional requirements. For many enzymes, cytokines, growth factors, antibody fragments and other non-glycosylated proteins, microbial expression provides an efficient route to development and manufacturing, combining rapid timelines with high productivity and cost-effective scale-up.
Choosing the right expression system early can significantly reduce development risks and accelerate your path to the clinic or the market. Our scientific team helps evaluate your molecule, manufacturing goals and quality requirements to determine whether a microbial platform is the optimal solution.
The complete microbial pipeline — select any stage to explore that service in detail.
Explore Our Microbial Services
Strain Development
Experience with E. coli and P. pastoris but also many other microbial strains like Bacillus and fungal expression systems — developing high-yielding production strains whether you need fast prototyping, yield improvement or complex protein expression.
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Cell Banking
A fully integrated solution for your microbial cell banking needs: Master and Working Cell Bank generation, characterization, long-term storage and release, with our team alongside you through every stage.
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Process Development
Deep upstream and downstream expertise to tackle challenges such as low expression, inclusion bodies and downstream bottlenecks — with a flexible operation structure adapted to your protein and expression system.
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Analytical Services
Integrated analytical services across raw-material testing, in-process control, release and stability studies. More than 90 already-validated methods reduce outsourcing and keep analytical data connected to manufacturing decisions.
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Protein Manufacturing
Scale microbial protein production from preclinical to clinical and commercial supply, GMP or non-GMP. We combine 200 L and 2000 L GMP capacity with non-GMP production up to 500 L, so you choose the quality level that matches your program stage.
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Fill & Finish
Complete your program with aseptic Fill & Finish. We support Drug Product manufacturing in vials, bottles and single-use bags, with long-term storage and batch-release documentation for preclinical, clinical and selected commercial needs.
Learn MoreTypical Recombinant Proteins Produced Using Microbial Expression
| Protein Category | Typical Examples |
|---|---|
| Enzymes | In vitro transcription enzymes, in vitro diagnostic enzymes, therapeutic enzymes |
| Growth Factors | bFGF, EGF, VEGF, IGF variants |
| Cytokines | IL-2, IL-15, IFNs, TNF family |
| Antibody Fragments | scFv, Fab, nanobodies |
| Scaffold Proteins | Affibodies, DARPins, alternative scaffolds |
| Plasmid-encoded Proteins | Vaccine antigens and recombinant proteins |
Every biologic is unique. Our scientific team works with you to evaluate the most suitable expression platform based on your protein, development objectives and manufacturing requirements.
Why Protein Production Is Different When You Do It With 53Biologics
We Don't Step Back. We Step Up.
With fully integrated in-house capabilities and direct scientist-to-client engagement, we remove the barriers that typically sit between your program and its progress.
We Earn Trust by Delivering, Not Promising
53Biologics brings the science and accountability needed when the stakes are high. You can progress with confidence, knowing quality is protected and continuity is maintained from one stage to the next.
Built Around Your Program
With experience across diverse biologics platforms and integrated capabilities that can be tailored at each stage, we give clients the flexibility to find the best fit at every step.
We Succeed When You Do
You need a partner who treats your success as their own, not just a set of tasks to complete. 53Biologics shares ownership of the outcome and stays engaged from the first decision to final delivery.
Request a Quote for Your Microbial Protein Program
Tell us about your protein and manufacturing goals — our scientific team will get back to as soon as possible.
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