Protein Manufacturing Services

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Your Protein, Our Microbial Expertise

53Biologics offers custom packages to produce your recombinant protein efficiently, from development to clinical stages. We provide robust and reproducible production services adapting to the required quality standard: non-GMP, GMP-like, or full GMP.

What Quality Grade Do You Need for your Microbial Protein?

If you need GMP…

You are in need of GMP production for your therapeuctic protein (i.e. enzymes, antigens, viral subunits, growth factors, cytokines)

If you need GMP-like/ISO 13485…

Your current needs relies on ancillary reagents like enzymes for diagnostic, enzymes for in vitro transcription,, gene therapy supporting enzymes and other bioprocessing enzymes.

Capacities for Microbial Protein Production

GMP Line 1

Up to 200 L

USP: from 20 L to 200 L stainless steel bioreactors

DSP: centrifuge, HPH, TFF and chromatography 

Ideal for preclinical and early clinical phases

GMP Line 2

up to 2000 L

USP: 200 L and 2000 L stainless steel bioreactors

DSP: centrifuge, HPH, TFF and chromatography 

Able to handle demands of complex and high-volume productions

Non-GMP Line

up to 500 L

USP: 100 L and 500 L stainless steel bioreactors

DSP: centrifuge, HPH, iltration skids and holders and chromatography 

Perfect for early stage or scale-up needs

Drug Product Manufacturing and Release

Alongside drug substance production, we offer integrated drug product manufacturing services, including aseptic fill & finish for clinical and commercial supply. Our capabilities support a seamless transition from bulk drug substance to ready-to-use drug product.

  • Aseptic filling performed in accordance with EU GMP Annex I
  • Scalable batch sizes up to 5,000 vials, 600 bottles and 500 bags 
  • Drug product formats include vials, bottles and single-use bags (250 mL – 200 L)
  • Comprehensive quality control testing and GMP batch release

Regulatory Expertise

  • EMA-inspected GMP facilities, manufacturing authorization ES/091/21
  • +25 successful audits in the last 2 years
  • +15 GMP batches released annually
  • Robust QC system with +90 validated methods available

At 53Biologics, we provide dedicated microbial manufacturing lines designed to support recombinant protein projects at every stage, from early non-GMP runs to full GMP supply. We guarantee a reproducible level of quality for all of your productions thanks to our in-house analytical QC capabilities and our skilled QA team.

Why Choose 53Biologics for your Microbial Production?

IP-free Strains

We do not charge royalties or fees for the use of our microbial strains. 

Flexible Scales

GMP manufacturing scales of 200 L and 2000 L with DSP equipment associated capable of  handling batches from mg to kg.

End-to-end Support

End-to-end DS and DP services with a single partner, minimizing transfer costs to different partners.

Fast Timelines

Thanks to our lean operations and proactive team, project kick-off starts sooner, reducing delays compared to competitors.

Track Record

Experience producing microbial proteins such us biotherapeutic proteins, VLP, enzymes, growth factors and more.

GMP and GMP-like Available

We can perform GMP-like under ISO 13485, and full GMP in accordance with EU GMP.

High quality services for non-GMP needs

We know that your project still needs a high degree of quality to ensure process and product consistency even when GMP compliance is not demanded.

100 L

Stainless steel biorector

500 L

Stainless steel biorector

Discover more about our CDMO protein services

Interested In Our Protein Services?

We would love to hear from you!

Please note that job applications submitted through this form will not be considered. Use the Careers page.

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