Home › Proteins › Microbial › Strain Development
Microbial Strain Development
Trust in our ability to developed and optimized strains of E. coli, P. pastoris and other microbial hosts like B. subtilis or filamentous fungi. We tailor each strain to the specific requirements of your process.
Strain development is the starting point of our integrated microbial pipeline. We build the production strain your entire program will rely on, with every later stage in mind.
01
Strain Development
02
Cell Banking
03
Process Development
04
Analytical Support
05
Manufacturing
06
Fill & Finish
This service is available also for:
Overview
Expression Systems Built Around Your Protein
Choosing the right microbial host is one of the most critical decisions in recombinant protein production. We develop and optimize expression systems using Escherichia coli, Pichia pastoris and other microbial host (i.e. fungal platforms, Bacillus subtilis…). The selection of the best host is based on the advantages of every host and your protein complexity.
Our Expertise in Microbial Hosts
Escherichia coli
Use a fast, well-characterized host for non-glycosylated proteins and cost-efficient development. At 53Biologics we harness the versatility of E. coli as a microbial host, combining strains and optimized plasmids to support efficient recombinant protein production.
• High Productivity
• Ideal for non-glycosylated proteins
• IP-free toolkits available to reduce licensing costs
Pichia pastoris
Apply yeasts expression systems for efficient protein secretion, high-density cultivation and scalable production. Our expertise in yeast expression systems, particularly P. pastoris, allows us to produce recombinant proteins with high yield and scalability.
• Ability to secret proteins
• No endotoxin concerns
• IP-free toolkits available to reduce licensing costs
Others
Explore Bacillus and filamentous fungal hosts to identify the most suitable expression platform for your protein. We work with Bacillus strains as well as established fungal systems, including Trichoderma, Aspergillus and Myceliophthora thermophila (C1), leveraging their distinct capabilities for protein secretion, productivity and process performance.
• Bacillus enable efficient protein secretion
• Fungal strains are suitable for complex protein
• We have expertise to guide host selection
Workflow
Our Microbial Strain Development Workflow
01
Design of
Prototypes
Design of plasmids combining promoters, signal peptides, tags, etc.
02
Strain engineering & Screening
Selection of the most suitable cells and Research Cell Bank (RCB) development.
03
Cell bank
Generation
RCB or MCB/WCB generation and characterization with long-term storage available
04
Subsequent Development and Manufacturing
Process development, scale-up and manufacturing readiness
Why Choose 53Biologics for Strain Development
Built Around Your Expression System
Every microbial expression system presents different technical challenges. We tailor the strain development strategy to your protein, construct and production goals to create the strongest possible starting point.
Scientific Expertise That Delivers
Our teams not only combine strain engineering and molecular biology knowledge but also process development and manufacturing knowledge. This facilitates a decision-making process that supports both expression performance and subsequent scalability.
Work
Side-by-Side
Your scientists work directly with the people developing your strain, enabling fast communication, shared technical decisions and close collaboration throughout the program. From first talk to the final delivery, we work as an extension of your team.
A Partner Beyond Strain Development
We stay involved as your program progresses, providing the technical continuity needed to move confidently from strain development into process development, manufacturing and long-term production.
Support
Frequently Asked Questions
Common questions about our microbial strain development services. Can’t find what you need? Talk to our team.
Can existing microbial strains be transferred to 53Biologics?
Yes. We can seamlessly transfer client-developed microbial strains into our development and manufacturing platform. Alternatively, if a production strain has not yet been established, we can support host selection, construct design and strain development to build an expression system tailored to your protein and manufacturing goals.
Do I need to have my expression construct before starting a project?
No. We can work from an existing construct or support vector design, gene optimization and cloning to create an expression system tailored to your project.
How do you select the best expression strategy?
When multiple hosts are an option, we work with you in a short host-selection project, evaluating protein production across the different microbial strains (expression levels, solubility and more) to recommend the most appropriate host and construct design.
Can strain development be performed with IP-free tools?
Yes, when technically appropriate. Depending on the target protein, selected host and project requirements, we can use IP-free molecular biology tools to support construct design and strain development, helping reduce licensing complexity where feasible.
What information is needed to start a strain development project?
Typical starting information includes the gene sequence, target protein, intended application, preferred expression host (if known) and any existing expression or process data. Our team will help define the most suitable development strategy.
Request a Quote for Your Strain Development Project
Tell us about your protein and target host — our team will get back to you as soon as possible.
Latest News
Latest From Our Blog
Insights, protocols and perspectives from our scientific team.